The Africa One Health University Network (AFROHUN) held its 4th International One Health Conference at the Emara Ole Sereni Convention Centre in Nairobi, Kenya, from April 24th to 26th, 2024. The conference, theme was “The Role of Regional One Health University Networks in Advancing Global Health Security” and gathered over 300 global One Health practitioners, scientists, students, and young professionals. It focused on addressing One Health challenges related to climate change and antimicrobial resistance (AMR).